Cat #: BLP-PC057
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-HCN3 Antibody (#APC-057). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of HCN3 in rat brain lysate:1. Anti-HCN3 Antibody (#APC-057), (1:200).
2. Anti-HCN3 Antibody, preincubated with HCN3 Blocking Peptide (#BLP-PC057).
- Expression of HCN3 in rat dorsal root ganglia (DRG)Immunohistochemical staining of frozen section of rat DRG using Anti-HCN3 Antibody (#APC-057), (1:50) followed by mouse anti-rabbit Alexa Fluor 555 (red). Staining is present in neuronal cell bodies. Hoechst 33342 (blue) is used as counterstain.
- (C)QRATGDGSPRRKGSGSER corresponding to amino acid residues 727-744 of rat HCN3 (Accession Q9JKA8).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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