Anti-HCN3 Antibody

K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
    Cat #: APC-057
    Alternative Name K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)QRATGDGSPRRKGSGSER corresponding to amino acid residues 727-744 of rat HCN3 (Accession Q9JKA8). Intracellular, C-terminus.
    • Anti-HCN3 Antibody
    Accession (Uniprot) Number Q9JKA8
    Gene ID 114245
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse - identical.
    RRID AB_2039904.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-HCN3 Antibody
      Western blot analysis of HCN3 in rat brain lysate:
      1. Anti-HCN3 Antibody (#APC-057), (1:200).
      2. Anti-HCN3 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-HCN3 Antibody
      Expression of HCN3 in rat dorsal root ganglia (DRG)
      Immunohistochemical staining of frozen section of rat DRG using Anti-HCN3 Antibody (#APC-057), (1:50) followed by mouse anti-rabbit Alexa Fluor 555 (red). Staining is present in neuronal cell bodies. Hoechst 33342 (blue) is used as counterstain.
    References
    1. Much, B. et al (2003) J. Biol. Chem. 278, 43781.
    2. Notomi, T. and Shigemoto R. (2004) J. Comp. Neurol. 471, 241.
    3. IUPHAR: http://www.iuphar-db.org/iuphar-rd/index.html.
    Scientific background

    Hyperpolarization-activated cation currents (Ih) appear in the heart and brain and play a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2

    The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which comprises of four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2

    The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers.2

    The channels are closely related to each other and share homology of about 60%. However, they differ from each other in their cytoplasmic N- and the C-terminus. HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2HCN3 and HCN4.2,3

    The HCN3 channels are abundant in the brain where high expression of HCN3 mRNA was found in the hypothalamus.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-HCN3 Antibody (#APC-057) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize HCN3 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Immunohistochemistry citations
    1. Rat diaphragm sections (1:25).
      Negrini, D. et al. (2016) Am. J. Physiol. 311, H892.
    More product citations
    1. Hughes, D.I. et al. (2013) Neuroscience 237, 7.
    2. Nakashima, N. et al. (2013) J. Physiol. 591, 1749.
    3. Klueva, J. et al. (2012) Neurosignals 20, 35.
    4. Weng, X. et al. (2012) Pain 153, 900.
    5. Neitz, A. et al. (2011) Eur. J. Neurosci. 33, 1611.
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