Overview
Type: Synthetic peptide
Form: Lyophilized powder
Histamine H2 Receptor/HRH2 (extracellular) Blocking Peptide (#BLP-HR002) is the original antigen used for immunization during Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Histamine H2 Receptor/HRH2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat basophilic leukemia cells (RBL) (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1,2. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:200).
3,4. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody, preincubated with Histamine H2 Receptor/HRH2 (extracellular) Blocking Peptide (#BLP-HR002).
Expression of Histamine H2 receptor in rat stomachImmunohistochemical staining of paraffin embedded section of rat stomach using Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:100). H2R (brown staining) is expressed in parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.
Properties
Sequence
- RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat HRH2 (Accession P25102).
Accession (Uniprot) Number P25102
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
