Certificate of Analysis

Human NGF Premium Research Pack

All You Need for NGF Research
Cat #: ESP-650 10 Vials

Antibody Compounds

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-NGF Antibody Cat #: AN-240

Size: 0.2 ml Source: Rabbit Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Highly purified 2.5 S mouse NGF (Accession P01139).

Mouse NGF/proNGF Neutralizing Antibody Cat #: ALM-006

Size: 0.25 mg Source: Mouse Type: Monoclonal Application: IE, N Reactivity: H, M
Immunogen
Highly purified 2.5S mouse NGF (Accession P01139).

Anti-p75 NGF Receptor (extracellular) Antibody Cat #: ANT-007

Size: 0.2 ml Source: Rabbit Type: Polyclonal Application: IC, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.
Homology
Mouse - identical; rat - 15/16 amino acid residues identical.

Anti-TrkA (extracellular) Antibody Cat #: ANT-018

Size: 0.2 ml Source: Rabbit Type: Polyclonal Application: IC, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA (Accession P35739). 2nd extracellular immunoglobulin-like domain.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.

Non-Antibody Compounds

Recombinant human beta-NGF protein Cat #: N-245

Size: 50 µg Source: Recombinant, E. coli MW: 27 kDa. Purity: >98% (HPLC) Net peptide content: 100%
Effective concentration
EC50 = 0.4 nM.
Activity
NGF is purified in three forms: the 7S, 2.5S and β. The biologically active subunit is the β, which is a 26 kDa. dimer composed of two identical chains held together by hydrophobic interactions1. NGF has been shown to regulate neuronal survival, development function and plasticity2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water, DMEM at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

GW441756 hydrochloride Cat #: G-191

Size: 25 mg Source: Synthetic MW: 311.8 Purity: ≥97%
Effective concentration
2 nM - 2 µM.
Activity
GW441756 is a potent, selective inhibitor of the NGF receptor tyrosine kinase A (TrkA)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
4 – 10 mM in H2O. Centrifuge all product preparations before use (5,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

K252a Cat #: K-150

Size: 0.1 mg Source: Natural MW: 467.5 Purity: >98%
Origin
Nocardiopsis sp. (soil fungi).
Effective concentration
10-200 nM.
Activity
K252a inhibits a variety of kinases including PKC (IC50 = 25 nM), PKA (IC50 = 18 nM) and PKG (IC50 = 20 nM)1. It also inhibits CaMK (IC50 = 1.8 nM) and MLCK (IC50 = 17 nM). K252a has also been shown to inhibit NGF mediated proliferation through Trk receptor family2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.
Protect from light.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

Note
A mouse monoclonal antibody (#ALM-006) is included in this Research Pack. Please take into account when reacting with a secondary antibody.