Lyophilized Powder Lyophilized Powder
This product is freeze dried. All water molecules have been removed.
Bioassay Tested Bioassay Tested
The activity of this product has been verified and approved by our professional team.
Our Bioassay
Source Synthetic
MW: 627.78
Purity: >95%
Effective concentration 0.1-1 µM.
Chemical name (2Z)-4-[(1S,2S,8R,17S,19R)-12-Hydroxy-8,21,21-trimethyl-5-(3-methyl-2-buten-1-yl)-8-(4-methyl-3-penten-1-yl)-14,18-dioxo-3,7,20-trioxahexacyclo[15.4.1.02,15.02,19.04,13.06,11]docosa-4(13),5,9,
 11,15-pentaen-19-yl]-2-methyl-2-butenamide.
Molecular formula C38H45NO7.
CAS No.: 286935-60-2.
Activity Gambogic amide, an NGF-mimetic small molecule, is a TrkA receptor agonist, shown to selectively bind, activate and upregulate the expression of TrkA receptors at nanomolar concentrations (Kd = 75 nM). Gambogic amide shows antiapoptotic activity (EC50 = 5 nM), neuroprotective and anti-cancer effects in vitro and in vivo1-3.
References - Activity Jang, S.W. et al. (2007)
Proc. Natl. Acad. Sci. U.S.A. 104, 16329.
Chan, C.B. et al. (2009)
Oncogene 28, 3825.
Shen, J. and Yu, Q. (2015)
Pharmacol. Rep. 67, 217.
Storage before reconstitution Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution Soluble up to 100 mM in DMSO. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution -20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.
Our bioassay Alomone Labs Gambogic amide inhibits the proliferation of K562 human chronic myelogenous leukemia cells.
Dose-response curve of Gambogic amide (#G-235) inhibition of K562 cell viability. Cell viability was determined by the XTT method and normalized to control. IC50 was calculated at 204 nM.
References - Scientific Background Jang, S.W. et al. (2007)
Proc. Natl. Acad. Sci. U.S.A. 104, 16329.
Chan, C.B. et al. (2009)
Oncogene 28, 3825.
Shen, J. and Yu, Q. (2015)
Pharmacol. Rep. 67, 217.
Scientific background Gambogic amide, is a small molecule that mimics nerve growth factor (NGF) and is a selective agonist of TrkA receptor that strongly induces its tyrosine phosphorylation and downstream signaling activation.
Gambogic amide interacts with the cytoplasmic juxtamembrane domain of TrkA receptor and triggers its dimerization. It selectively binds, activates and upregulates the expression of TrkA receptors at nanomolar concentrations but not TrkB or TrkC.
Gambogic acid is a major active ingredient of gamboge, a resin exuded from the Garcinia hanburryi tree in southeast Asia. Gambogic amide also possesses neurotrophic activity in various neural injury animal models and prevents glutamate-induced neuronal cell death1,2.
Gambogic amide shows antiapoptotic activity and demonstrates EC50 value of 5 nM3.
Target TrkA receptors
Lyophilized Powder
Last update: 22/11/2018
Gambogic amide (#G-235) is a highly pure, synthetic, and biologically active compound.
For research purposes only, not for human use
