Human PAR1/F2R (extracellular) Blocking Peptide (#BLP-PR031) is the original antigen used for immunization during Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031) generation. The blocking peptide binds and ‘blocks’ Anti-Human PAR1/F2R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human promyelocytic leukemia HL-60 (lanes 1 and 4), human T-cell leukemia Jurkat (lanes 2 and 5), and chronic myelogenous leukemia K562 (lanes 3 and 6) cell line lysates:
- Western blot analysis of human colon cancer HT-29 (lanes 1 and 3) and human Colo-205 colon cancer (lanes 2 and 4) cell line lysates:
- Expression of PAR-1 in normal human breast and human breast carcinomaImmunohistochemical staining of paraffin-embedded human breast sections using Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:100). PAR-1 staining is highly specific for epithelium-derived cells. A. In the normal resting breast, epithelial cells of the mammary ducts are visible using Histofine (pink). B. The breast carcinoma contains epithelium-derived malignant cells stained with DAB (brown). Hematoxilin is used as the counterstain.
- (C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1 (Accession P25116).