Human PAR2/F2RL1 (extracellular) Blocking Peptide (#BLP-PR035) is the original antigen used for immunization during Anti-Human PAR2/F2RL1 (extracellular) Antibody (#APR-035) generation. The blocking peptide binds and ‘blocks’ Anti-Human PAR2/F2RL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 6), human PANC-1 pancreatic carcinoma cell line lysate (lanes 2 and 7), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 8), human Jurkat T-cell leukemia cell line lysate (lanes 4 and 9) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 5 and 10):
- (C)GKVDGTSHVTGKG, corresponding to amino acid residues 40 - 52 of human PAR2/F2RL1 (Accession P55085).