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Human TrkC-Fc Chimera

An Inhibitor of NT-3 Activity

Cat #: RPC-004
Lyophilized Powder yes
  • Bioassay Tested
    • Sterile & Endotoxin Free yes
    Origin Recombinant, HEK 293-6E cells.
    MW: ~ 100 kDa.
    Endotoxin Level <0.1 EU per 1 µg of the protein by the LAL method.
    Purity: >90% (HPLC)
    Form The product is lyophilized from a 0.2 μm filtered solution in PBS.
    Effective concentration 0.2-0.5 µg/ml in the prescence of 30 ng/ml of human NT-3.
    Sequence Human TrkC (Cys32-Asp428)-DIEGEMD-Human IgG1 (Pro100-Lys330).
    Modifications Glycosylation.
    Activity Human TrkC-Fc Chimera inhibits NT-3-induced phosphorylation of ERK1/2 in HEK293 cells expressing TrkB receptors.
    References-Activity
    1. Bouzas-Rodriguez, J. et al. (2010) J. Clin. Invest. 120, 850.
    2. Palazzo, E. et al. (2012) J. Cell. Physiol. 227, 1017.
    3. Ramos, B. et al. (2009) Mol. Cell. Neurosci. 42, 152.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Centrifuge (10000 x g 5 min) all product preparations before use.
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Human TrkC-Fc Chimera inhibits NT-3-induced ERK1/2 MAPK phosphorylation in TrkB stably expressed in HEK293 cells.
      Alomone Labs Human TrkC-Fc Chimera inhibits NT-3-induced ERK1/2 MAPK phosphorylation in TrkB stably expressed in HEK293 cells.
      TrkB-stably transfected HEK293 cells were serum deprived for 2 hr and then stimulated for 10 min with 30 ng/ml Recombinant human Neurotrophin (NT-3) protein (#N-260) (lane 2) or 0.5 µg/ml Human TrkC-Fc Chimera (#RPC-004) preincubated with 30 ng/ml Recombinant human Neurotrophin (NT-30 protein for 90 min (lane 3). Control cells incubated without human NT-3 are shown in lane 1. Cell proteins were resolved by SDS-PAGE and detected with anti-phospho-ERK1/2.
    References - Scientific background
    1. Ramos, B. et al. (2009) Mol. Cell. Neurosci. 42, 152.
    2. Green, S.H. et al. (2012) Anat. Rec. 295, 1877.
    3. Huang, E.J. et al. (2003) Annu. Rev. Biochem. 72, 609.
    4. Reichardt, L.F. (2006) Philos. Trans. R. Soc. Lond. B Biol. Sci. 361, 1545.
    Scientific background

    TrkC-Fc chimera is a recombinant protein, a soluble fusion of the TrKC receptor which comprises the extracellular domains of the human TrkC fused to the IgG heavy chain. TrkC-Fc has the binding specificity and affinity of TrkC receptor that can block the biological activity of neurotrophin-31.

    The neurotrophin family includes four members- nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These are encoded by NGF, BDNF, NTF3, and NTF4 human genes, respectively2.

    Neurotrophins activate in part Trk family receptor protein-Tropomyosin related kinases. The Trk family includes three members: TrkA, TrkB, and TrkC, encoded by NTRK1, NTRK2, and NTRK3 human genes, respectively. TrkC has the highest affinity for NT-32.

    The Trk structure includes a cytoplasmic domain containing several sites of tyrosine phosphorylation which are responsible for activating intracellular signaling cascades. The extracellular domain consists of a cysteine-rich cluster followed by three leucine-rich repeats, another cysteine-rich cluster and two immunoglobulin-like domains3,4.

    Target NT-3
    Net Peptide Content: 100%
    Lyophilized Powder
    Last update: 02/03/2021

    Human TrkC-Fc Chimera (#RPC-004) is a highly pure, recombinant, and biologically active protein.

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    For research purposes only, not for human use

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