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Anti-Neurotrophin 3 (NT-3) Antibody

NTF3, Nerve growth factor 2, NGF2, HDNF

Cat #: ANT-003
Alternative Name NTF3, Nerve growth factor 2, NGF2, HDNF
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor) (Accession P20783).
Accession (Uniprot) Number P20783
Gene ID 4908
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, chicken, Xenopus - identical; cat - 9/10 amino acid residues identical.
RRID AB_2040013.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.3 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
Immunohistochemistry
  • Expression of Neurotrophin 3 in mouse spinal cord
    Expression of Neurotrophin 3 in mouse spinal cord
    Immunohistochemical staining of perfusion-fixed frozen mouse spinal cord sections with Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 (green). The same sections were stained with mouse anti-neurofilament 200 (NF) (red). Merge of the two images demonstrates the presence of NT-3 in gray matter which colocalizes with NF only in the soma of some neurons (arrow).
  • Rat hippocampus (1:300) (Ramos Languren, L.E. and Escobar, M.L. (2013) Eur. J. Neurosci. 37, 1248).
References
  1. Roux, P.P. and Barker, P.A. (2002) Prog. Neurobiol. 67, 203.
  2. McDonald N.Q. et al. (1991) Nature 354, 411.
  3. Arvanian, V.L. et al. (2003) J. Neurosci. 23, 8706.
  4. Tzeng, S.F. and Huang, H.Y. (2003) J. Cell Biochem. 90, 227.
  5. Sekimoto, M. et al. (2003) Immunol. Lett. 88, 221.
  6. Kimitsuki, T. et al. (2003) Auris. Nasus Larynx. 30, 141.
  7. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.
Scientific background

The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.2 The rat and human forms of neurotrophin-3 (NT-3) are 96% homologous.  

NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,3 to serve as an anti-inflammatory factor, to suppress microglial activation,4 to play a critical role in regulating T helper 1/T helper 2 cell balance5 and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.6 The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3, and p75NTR, which binds all the neurotrophins.7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 06/11/2022

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Neurotrophin 3. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003) can be used in western blot and immunohistochemical applications. It has been designed to recognize NT-3 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Mouse spinal cord sections (1:200).
    Kumar, H. et al. (2020) J. Neurosci. 40, 1943.
  2. Rat hippocampus (1:300).
    Ramos-Languren, L.E. and Escobar, M.L. (2013) Eur. J. Neurosci. 37, 1248.
Immunofluorescence citations
  1. Mouse spinal cord sections (1:200).
    Kumar, H. et al. (2020) J. Neurosci. 40, 1943.

Specifications

Scientific Background

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