Overview
Cat #: BLP-CC019
Type: Synthetic peptide
Form: Lyophilized powder
IP3 Receptor-1/ITPR1 Blocking Peptide (#BLP-CC019) is the original antigen used for immunization during Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019) generation. The blocking peptide binds and ‘blocks’ Anti-IP3 Receptor-1/ITPR1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membranes:1. Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019), (1:2000).
2. Anti-IP3 Receptor-1 (ITPR1) Antibody, preincubated with IP3 Receptor-1/ITPR1 Blocking Peptide (#BLP-CC019). - Expression of IP3R1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum with Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019). Immunoreactivity appears in Purkinje cells (arrows in A) and their dendritic trees. In addition, axonal processes coursing through cerebellar white matter are visualized (arrow in B).
Properties
Sequence
Accession (Uniprot) Number P29994
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.