IQGAP1 Blocking Peptide (#BLP-PZ055) is the original antigen used for immunization during Anti-IQGAP1 Antibody (#APZ-055) generation. The blocking peptide binds and ‘blocks’ Anti-IQGAP1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 4):
- Expression of IQGAP1 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-IQGAP1 Antibody (#APZ-055), (1:200), followed by goat anti-rabbit-AlexaFluor-488. IQGAP1 immunoreactivity appears in Purkinje neurons (horizontal arrows) and their dendrites (vertical arrows). Cell nuclei are stained with DAPI (blue).
- (C)KAYQDRLAYLRSHKDE, corresponding to amino acid residues 793 - 808 of human IQGAP1 (Accession P46940).