KChIP2 Blocking Peptide (#BLP-PC142) is the original antigen used for immunization during Anti-KChIP2 Antibody (#APC-142) generation. The blocking peptide binds and ‘blocks’ Anti-KChIP2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate:
- Western blot analysis of rat heart (lanes 1 and 3) and rat hippocampus (lanes 2 and 4) lysate:
- Expression of KChIP2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-KChIP2 Antibody (#APC-142), (1:400), followed by goat-anti-rabbit-Alexa-488 (green). KChIP2 staining in the hippocampal CA1 region appears in neuronal profiles (arrows) in the pyramidal layer (Pyr). DAPI is used to stain nuclei (blue).
- (C)RGQGRKESLSDSRD, corresponding to amino acid residues 2-15 of human KChIP2 (Accession Q9NS61).