Anti-KChIP2 Antibody

KCNIP2, KV channel-interacting protein 2, A-type potassium channel modulatory protein 2, Cardiac voltage-gated potassium channel modulatory subunit
    Cat #: APC-142
    Alternative Name KCNIP2, KV channel-interacting protein 2, A-type potassium channel modulatory protein 2, Cardiac voltage-gated potassium channel modulatory subunit
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RGQGRKESLSDSRD, corresponding to amino acid residues 2-15 of human KChIP2 (Accession Q9NS61). Cytoplasmic, N-terminus.
        Anti-KChIP2 Antibody
    Accession (Uniprot) Number Q9NS61
    Gene ID 30819
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical. Rat - 13/14 amino acid residues identical.
    RRID AB_2756744.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-KChIP2 Antibody
        Western blot analysis of mouse brain lysate:
        1. Anti-KChIP2 Antibody (#APC-142), (1:200).
        2. Anti-KChIP2 Antibody, preincubated with the negative control antigen.
        Anti-KChIP2 Antibody
        Western blot analysis of rat heart (lanes 1 and 3) and rat hippocampus (lanes 2 and 4) lysate:
        1,2. Anti-KChIP2 Antibody (#APC-142), (1:200).
        3,4. Anti-KChIP2 Antibody, preincubated with the negative control antigen.
      • Anti-KChIP2 Antibody
        Expression of KChIP2 in mouse hippocampus
        Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-KChIP2 Antibody (#APC-142), (1:400), followed by goat-anti-rabbit-Alexa-488 (green). KChIP2 staining in the hippocampal CA1 region appears in neuronal profiles (arrows) in the pyramidal layer (Pyr). DAPI is used to stain nuclei (blue).
    References
    • 1. Holmqvist, M.H. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 1035.
    • 2. Lundby, A. et al. (2010) Br. J. Pharmacol. 160, 2028.
    • 3. Rosati, B. et al. (2001) J. Physiol. (Lond.) 533, 119.
    • 4. Grubb, S. et al. (2012) Front. Physiol. 3, 118.
    • 5. Kuo, H.C. et al. (2001) Cell 107, 801.
      • KChIPs belong to the family of small cytosolic calcium-binding proteins and consist of four different KChIP isoforms ranging between 216 and 270 amino acids. They all have a reasonably conserved core region of 185 amino acids and a heterogeneous N-terminal. KChIPs have calcium-binding EF hands which appear to be important for modulation of KV channels but not for CaV channels1.

        The cardiac KChIP2 gene generates three protein isoforms of 220, 252, and 270 amino acids via alternative splicing of exons 2 and 3. It is well established that KChIP2 functionally increases peak KV4 current, slows channel inactivation and accelerates recovery from inactivation. Furthermore, KChIP2 has been reported to increase ICa,L and suggested to decrease KV1.5 cell surface expression and facilitate INa2 .

        All four KChIP mRNAs are found in the brain, however only KChIP2 is expressed in the heart3. KChIP2 is expressed heterogeneously across the canine and human left and right ventricular free wall with higher concentrations in epicardium compared to endocardium4.

        Mice deficient for KChIP2 exhibit normal cardiac structure and function but display a prolonged elevation in the ST segment on the electrocardiogram. The KChIP2−/− mice are highly susceptible to the induction of cardiac arrhythmias5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-KChIP2 Antibody (#APC-142) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KChIP2 from rat, mouse, and human samples.

    For research purposes only, not for human use