Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNE3/MiRP2 Blocking Peptide (#BLP-PC118) is the original antigen used for immunization during Anti-KCNE3 (MiRP2) Antibody (#APC-118) generation. The blocking peptide binds and ‘blocks’ Anti-KCNE3/MiRP2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and heart (lanes 2 and 4) membranes:1,2. Anti-KCNE3 (MiRP2) Antibody (#APC-118), (1:200).
3,4. Anti-KCNE3 (MiRP2) Antibody, preincubated with KCNE3/MiRP2 Blocking Peptide (#BLP-PC118).- Rat kidney sections.
Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.).
Properties
Sequence
- (C)RSRKVDKRSDPYH, corresponding to amino acid residues 81-93 of human KCNE3 (Accession Q9Y6H6).
Accession (Uniprot) Number Q9Y6H6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
