Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNE4/MiRP3 Blocking Peptide (#BLP-PC129) is the original antigen used for immunization during Anti-KCNE4 (MiRP3) Antibody (#APC-129) generation. The blocking peptide binds and ‘blocks’ Anti-KCNE4/MiRP3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat pancreas membrane (lanes 1 and 3) and heart (lanes 2 and 4) lysates:1,2. Anti-KCNE4 (MiRP3) Antibody (#APC-129), (1:600).
3,4. Anti-KCNE4 (MiRP3) Antibody, preincubated with KCNE4/MiRP3 Blocking Peptide (#BLP-PC129).
Properties
Sequence
- (C)GYMKSKRREKKSS, corresponding to amino acid residues 56-68 of mouse KCNE4 (Accession Q9WTW3).
Accession (Uniprot) Number Q9WTW3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
