Anti-KCNE4 (MiRP3) Antibody

K+ channel β subunit subfamily E member 4, MinK-related peptide 3
Cat #: APC-129
Alternative Name K+ channel β subunit subfamily E member 4, MinK-related peptide 3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)GYMKSKRREKKSS, corresponding to amino acid residues 56- 68 of mouse KCNE4 (Accession Q9WTW3). Intracellular, C-terminus.
    Accession (Uniprot) Number Q9WTW3
    Gene ID 57814
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 2 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip
      • Western blot analysis of rat pancreas membranes (lanes 1 and 3) and rat heart lysate (lanes 2 and 4):
        1-2. Anti-KCNE4 (MiRP3) Antibody (#APC-129), (1:600).
        3-4. Anti-KCNE4 (MiRP3) Antibody, preincubated with the control peptide antigen.
      • The K+ voltage-gated channel subfamily E member (KCNE) family is a group of small, non-conducting, single transmembrane domain proteins that associate with pore-forming potassium channel subunits to form mixed complexes with unique characteristics1.

        Five different KCNE proteins have been described (KCNE1-5)2. The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the α subunits1.

        MinK-related peptides (MiRPs) MiRP3, protein encoded by KCNE43. The importance of these proteins to normal physiology of the heart and nervous system is exposed by their association with clinical disorders such as congenital long QT syndrome, drug-induced cardiac arrhythmias, sensorineural deafness, and periodic paralysis4. In addition, MiRPs influence the normal physiology of endocrine and exocrine glands, intestinal secretion, and renal excretion. MiRP3 is found to co-localize with KV4.2 subunits that contribute to cardiac transient outward K+ currents5. Levels of the KCNE4 transcript in human cardiac ventricle are robust and increase in patients with congestive failure suggesting a regulatory function for MiRP3 in the heart6. In addition to regulating KV4.2, MiRP3 also regulates KCNQ1 and BK channels2,5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse KCNE4. Anti-KCNE4 (MiRP3) Antibody (#APC-129) can be used in western blot analysis. It has been designed to recognize MiRP3 from rat, mouse and human samples.

    For research purposes only, not for human use