KCNH1/EAG-1 Blocking Peptide (#BLP-PC104) is the original antigen used for immunization during Anti-KCNH1 (EAG-1) Antibody (#APC-104) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH1/EAG-1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and HEK-KCNH1 (lanes 2 and 4):1,2. Anti-KCNH1 (EAG-1) Antibody (#APC-104), (1:200).
3,4. Anti-KCNH1 (EAG-1) Antibody, preincubated with KCNH1/EAG-1 Blocking Peptide (#BLP-PC104).
- Paraffin embedded human gliomas (1:200) (Bai, Y. et al. (2013) Eur. J. Cancer 49, 710.).
- GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KCNH1 (Accession Q63472).