Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNH1/EAG-1 Blocking Peptide (#BLP-PC104) is the original antigen used for immunization during Anti-KCNH1 (EAG-1) Antibody (#APC-104) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH1/EAG-1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and HEK-KCNH1 (lanes 2 and 4):1,2. Anti-KCNH1 (EAG-1) Antibody (#APC-104), (1:200).
3,4. Anti-KCNH1 (EAG-1) Antibody, preincubated with KCNH1/EAG-1 Blocking Peptide (#BLP-PC104).- Paraffin embedded human gliomas (1:200) (Bai, Y. et al. (2013) Eur. J. Cancer 49, 710.).
Properties
Sequence
- GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KCNH1 (Accession Q63472).
Accession (Uniprot) Number Q63472
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
