KCNH6/erg2 Blocking Peptide (#BLP-PC114) is the original antigen used for immunization during Anti-KCNH6 (erg2) Antibody (#APC-114) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH6/erg2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (1 and 3) and cortex (2 and 4) lysate:
- Expression of KV11.2 (erg2) in rat hippocampusImmunohistochemical staining of KV11.2 in rat hippocampus using Anti-KCNH6 (erg2) Antibody (#APC-114). A. KV11.2 appears as diffuse staining (green) that defines the boundary of the reticular nucleus of thalamus (arrow). B. Staining with mouse anti-parvalbumin (PV, red) demonstrates both neuronal and diffuse staining. C. Confocal merge of KV11.2 and PV demonstrates overlap of the two markers labeling the reticular nucleus of the thalamus (orange).
- Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.).
- TLNFVEFNLEKHRS(C), corresponding to amino acid residues 185-198 of human KV11.2 (erg2) (Accession Q9H252).