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Anti-KCNH6 (erg2) Antibody

KV11.2, Potassium voltage-gated channel subfamily H member 6, Ether-a-go-go-related channel 2, Eag-related protein 2, ERG-2, hERG2

Cat #: APC-114
Alternative Name KV11.2, Potassium voltage-gated channel subfamily H member 6, Ether-a-go-go-related channel 2, Eag-related protein 2, ERG-2, hERG2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide TLNFVEFNLEKHRS(C), corresponding to amino acid residues 185-198 of human KV11.2 (erg2) (Accession Q9H252). Intracellular, N-terminal part.
Accession (Uniprot) Number Q9H252
Gene ID 81033
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, chicken - identical; mouse - 13/14 amino acid residues identical.
RRID AB_2039842.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.75 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain (1 and 3) and cortex (2 and 4) lysate:
    Western blot analysis of rat brain (1 and 3) and cortex (2 and 4) lysate:
    1,2. Anti-KCNH6 (erg2) Antibody (#APC-114), (1:200).
    3,4. Anti-KCNH6 (erg2) Antibody, preincubated with KCNH6/erg2 Blocking Peptide (#BLP-PC114).
Immunohistochemistry
  • Expression of KV11.2 (erg2) in rat hippocampus
    Expression of KV11.2 (erg2) in rat hippocampus
    Immunohistochemical staining of KV11.2 in rat hippocampus using Anti-KCNH6 (erg2) Antibody (#APC-114). A. KV11.2 appears as diffuse staining (green) that defines the boundary of the reticular nucleus of thalamus (arrow). B. Staining with mouse anti-parvalbumin (PV, red) demonstrates both neuronal and diffuse staining. C. Confocal merge of KV11.2 and PV demonstrates overlap of the two markers labeling the reticular nucleus of the thalamus (orange).
  • Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.).
References
  1. Shi. W.M. et al. (1997) J. Neurosci. 17, 9423.
  2. Wimmers, S. et al. (2001) Pflugers Arch. 441, 450.
  3. Schwarz, J.R. and Bauer, C.K. (2004) J. Cell. Mol. Med. 8, 22.
Scientific background

KV11.2 (erg2) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels. The erg subfamily includes the closely related proteins KV11.1 (erg1) and KV11.3 (erg3) that possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with all voltage-dependent K+ channels, the functional channel is a tetramer composed of four subunits. It has been suggested that the KV11 subfamily members can form functional heteromultimers within the subfamily.

KV11.2 produces currents characterized by strong inward rectification with slow activation and very rapid inactivation kinetics, which closely resemble those produced by the much studied channel KV11.1.

The expression of KV11.2 seems to be limited to the brain and the pituitary gland. The same is true for the KV11.3 protein, while KV11.1 is more widely expressed. 

From a pharmacological point of view, KV11.2 channels can be blocked by well characterized organic blockers such as the anti-arrhythmic drug E-4031. In addition, the peptide toxins Ergtoxin-1 and BeKm-1 block KV11.2 with different potencies.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Alomone Labs is pleased to offer Anti-KCNH6 (erg2) Antibody (#APC-114) directed against a highly conserved epitope of the human KV11.2 channel. The antibody can be used to detect KV11.2 in rat samples using both western blotting and immunohistochemistry applications.

For research purposes only, not for human use
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