Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNH7/erg3 Blocking Peptide (#BLP-PC112) is the original antigen used for immunization during Anti-KCNH7 (erg3) Antibody (#APC-112) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH7/erg3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-KCNH7 (erg3) Antibody (#APC-112), (1:300).
2. Anti-KCNH7 (erg3) Antibody, preincubated with KCNH7/erg3 Blocking Peptide (#BLP-PC112).
Expression of KV11.3 (erg3) in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-KCNH7 (erg3) Antibody (#APC-112). A. KV11.3 channel appears in glial processes (red). B. Staining of astrocytic fibers with mouse anti-glial fibrillary acidic protein (GFAP, green). C. Confocal merge of KV11.3 channel and GFAP demonstrates colocalization in the molecular layer but separate localization of these proteins at the Purkinje cell layer.- Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.).
Properties
Sequence
- CPEFLDLEKSKLKSKE, corresponding to amino acid residues 1108-1123 of rat KV11.3 (erg3) (Accession O54852).
Accession (Uniprot) Number O54852
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
