KCNH7/erg3 Blocking Peptide (#BLP-PC112) is the original antigen used for immunization during Anti-KCNH7 (erg3) Antibody (#APC-112) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH7/erg3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate:
- Expression of KV11.3 (erg3) in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-KCNH7 (erg3) Antibody (#APC-112). A. KV11.3 channel appears in glial processes (red). B. Staining of astrocytic fibers with mouse anti-glial fibrillary acidic protein (GFAP, green). C. Confocal merge of KV11.3 channel and GFAP demonstrates colocalization in the molecular layer but separate localization of these proteins at the Purkinje cell layer.
- Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.).
- CPEFLDLEKSKLKSKE, corresponding to amino acid residues 1108-1123 of rat KV11.3 (erg3) (Accession O54852).