KCNJ1/Kir1.1 Blocking Peptide (#BLP-PC001) is the original antigen used for immunization during Anti-KCNJ1 (Kir1.1) Antibody (#APC-001) generation. The blocking peptide binds and ‘blocks’ Anti-KCNJ1/Kir1.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney membranes:
- Expression of KCNJ1 in rat kidneyImmunohistochemical staining of rat kidney sections using Anti-KCNJ1 (Kir1.1) Antibody (#APC-001), (left). There is strong staining (red) of tubular epithelial cells in distal tubes. Note that no staining is observed in proximal tubules (arrow). Counterstain of cell nuclei appears blue. A negative control is shown (right).
- GST fusion protein with the sequence HNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDET DDTQM, corresponding to amino acids 342-391 of rat KCNJ1 (Accession P35560).