Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK13/THIK-1 (extracellular) Blocking Peptide (#BLP-PC121) is the original antigen used for immunization during Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK13/THIK-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121), (1:200).
2. Anti-KCNK13 (THIK-1) (extracellular) Antibody, preincubated with KCNK13/THIK-1 (extracellular) Blocking Peptide (#BLP-PC121).
Western blot analysis of mouse heart membranes:1. Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121) (1:200).
2. Anti-KCNK13 (THIK-1) (extracellular) Antibody, preincubated with KCNK13/THIK-1 (extracellular) Blocking Peptide (#BLP-PC121).
Expression of KCNK13 in rat kidneyImmunohistochemical staining in paraffin-embedded sections of rat kidney using Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121), (1:50). Staining (brown color) is specific for renal tubules while both glomeruli (G) and arterial walls are negative (red arrows). Following Tris-EDTA treatment for antigen retrieval, slides were incubated overnight at 4°C. Hematoxylin is used as the counterstain.
Expression of KCNK13 in mouse substantia nigra.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121), (1:200), followed by goat anti-rabbit-Alexa-488. A. KCNK13 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with KCNK13 (THIK-1) (extracellular) Blocking Peptide (#BLP-PC121), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EERLANFSRGHNLSRE, corresponding to amino acid residues 54-69 of rat KCNK13 (Accession Q9ERS0).
Accession (Uniprot) Number Q9ERS0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
