Anti-KCNK13 (THIK-1) (extracellular) Antibody

Potassium channel subfamily K member 13, Tandem pore domain halothane-inhibited K+ channel 1, K2P13.1
Cat #: APC-121
Alternative Name Potassium channel subfamily K member 13, Tandem pore domain halothane-inhibited K+ channel 1, K2P13.1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
      • Peptide (C)EERLANFSRGHNLSRE, corresponding to amino acid residues 54-69 of rat KCNK13 (Accession Q9ERS0). 1st extracellular loop.
    Accession (Uniprot) Number Q9ERS0
    Gene ID 64120
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 15/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
      • Western blot analysis of rat kidney membranes:
        1. Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121), (1:200).
        2. Anti-KCNK13 (THIK-1) (extracellular) Antibody, preincubated with the control peptide antigen.
        Western blot analysis of mouse heart membranes:
        1. Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121) (1:200).
        2. Anti-KCNK13 (THIK-1) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Expression of KCNK13 in rat kidney
        Immunohistochemical staining in paraffin-embedded sections of rat kidney using Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121), (1:50). Staining (brown color) is specific for renal tubules while both glomeruli (G) and arterial walls are negative (red arrows). Following Tris-EDTA treatment for antigen retrieval, slides were incubated overnight at 4°C. Hematoxylin is used as the counterstain.
      • KCNK13 (also named Tandem pore domain halotane inhibited K+ channel, THIK -1 or K2P13.1) is a member of the 2-pore (2P) domain K+ channels family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.1

        The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.

        K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. but are insensitive to classical K+ channel blockers.

        The KCNK13 channel is activated by arachidonic acid and inhibited by the volatile anesthetic halothane. The channel is relatively widely expressed in peripheral tissues such as kidney, heart, lung and liver but it is also expressed in brain.
        The physiological function of the KCNK13 channel is not clear but an involvement in the physiological response to reduced oxygen levels (hypoxia) has been proposed.2

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope in the 1st extracellular loop of the rat KCNK13 channel. Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121) can be used in western blot and immunohistochemical applications, and recognizes KCNK13 channel from mouse and rat samples.

    For research purposes only, not for human use