KCNK2/TREK-1 Blocking Peptide (#BLP-PC047) is the original antigen used for immunization during Anti-KCNK2 (TREK-1) Antibody (#APC-047) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK2/TREK-1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:1. Anti-KCNK2 (TREK-1) Antibody (#APC-047), (1:200).
2. Anti-KCNK2 (TREK-1) Antibody, preincubated with KCNK2/TREK-1 Blocking Peptide (#BLP-PC047).
- Rat hippocampus sections (Banerjee, A. et al. (2016) J. Neurochem. 138, 265.).
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- Expression of KCNK2 (TREK-1) in rat midbrainImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-KCNK2 (TREK-1) Antibody (#APC-047-GP), (1:400), followed by goat-anti-guinea pig-Cy3 antibody. TREK-1 staining (red) appears in neurons in the rat lateral substantia nigra (arrows). Nuclei are stained with DAPI (blue).
- DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 (Accession O95069).