Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK4/TRAAK Blocking Peptide (#BLP-PC108) is the original antigen used for immunization during Anti-KCNK4 (TRAAK) Antibody (#APC-108) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK4/TRAAK primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of HEK-293-KCNK4 transfected cells:1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).
2. Anti-KCNK4 (TRAAK) Antibody, preincubated with KCNK4/TRAAK Blocking Peptide (#BLP-PC108).
Western blot analysis of rat cerebellum lysate:1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).
2. Anti-KCNK4 (TRAAK) Antibody, preincubated with KCNK4/TRAAK Blocking Peptide (#BLP-PC108).
Expression of KCNK4 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-KCNK4 (TRAAK) Antibody (#APC-108 ). A. KCNK4 channel appears in Purkinje neuronal processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK4 channel and calbindin D28K demonstrates the co-localization of these proteins.
Properties
Sequence
- NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human KCNK4 (Accession Q9NYG8).
Accession (Uniprot) Number Q9NYG8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Shipping and Ordering information
Need Help With This Product?
Our reagent specialists are here to help you find the best product for your application. Please call or email us and we will be happy to help you find the right product for the job.
Call us at
+972 2587 2202 or Email Us
