Anti-KCNK4 (TRAAK) Antibody

Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1
    Cat #: APC-108
    Alternative Name Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human KCNK4 (Accession Q9NYG8). Intracellular, C-terminus.
    • Anti-KCNK4 (TRAAK) Antibody
    Accession (Uniprot) Number Q9NYG8
    Gene ID 50801
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, dog - identical.
    RRID AB_2039951.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-KCNK4 (TRAAK) Antibody
      Western blot analysis of HEK-293-KCNK4 transfected cells:
      1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).
      2. Anti-KCNK4 (TRAAK) Antibody, preincubated with the negative control antigen.
    • Anti-KCNK4 (TRAAK) Antibody
      Western blot analysis of rat cerebellum lysate:
      1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).
      2. Anti-KCNK4 (TRAAK) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-KCNK4 (TRAAK) Antibody
      Expression of KCNK4 in rat cerebellum
      Immunohistochemical staining of rat cerebellum using Anti-KCNK4 (TRAAK) Antibody (#APC-108 ). A. KCNK4 channel appears in Purkinje neuronal processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK4 channel and calbindin D28K demonstrates the co-localization of these proteins.
    References
    1. Lesage, F. et al. (2000) FEBS Lett. 471, 137.
    2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793.
    3. Kim, D. (2003) Trends Pharmacol. Sci. 24, 648.
    Scientific background

    KCNK4 (also named TWIK-related arachidonic acid stimulated K+ channel, TRAAK or K2P4.1) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 16 members. These channels show little time- or voltage-dependence and are considered to be “leaky” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini.

    K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK4 can be integrated to a K2P subfamily that includes K2P2.1 (TREK1) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid, high intracellular pH and mechanical stretch.

    KCNK4 expression is largely confined to the brain in mouse samples while in humans it is also expressed in placenta and to a lesser degree in kidney, small intestine and prostate.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-KCNK4 (TRAAK) Antibody (#APC-108) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK4 from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    More product citations
    1. de la Pena, E. et al. (2012) PLoS ONE 7, e52475.
    2. Wu, X. et al. (2012) Int. J. Urol. 19, 944.
    3. Hur, C.G. et al. (2009) Reproduction 137, 237.
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