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Anti-KCNK4 (TRAAK) Antibody

Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1
Cat #: APC-108
Alternative Name Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human KCNK4 (Accession Q9NYG8). Intracellular, C-terminus.
Accession (Uniprot) Number Q9NYG8
Gene ID 50801
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, dog - identical.
RRID AB_2039951.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of HEK-293-KCNK4 transfected cells:
    Western blot analysis of HEK-293-KCNK4 transfected cells:
    1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).
    2. Anti-KCNK4 (TRAAK) Antibody, preincubated with KCNK4/TRAAK Blocking Peptide (#BLP-PC108).
  • Western blot analysis of rat cerebellum lysate:
    Western blot analysis of rat cerebellum lysate:
    1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).
    2. Anti-KCNK4 (TRAAK) Antibody, preincubated with KCNK4/TRAAK Blocking Peptide (#BLP-PC108).
Immunohistochemistry
  • Expression of KCNK4 in rat cerebellum
    Expression of KCNK4 in rat cerebellum
    Immunohistochemical staining of rat cerebellum using Anti-KCNK4 (TRAAK) Antibody (#APC-108 ). A. KCNK4 channel appears in Purkinje neuronal processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK4 channel and calbindin D28K demonstrates the co-localization of these proteins.
References
  1. Lesage, F. et al. (2000) FEBS Lett. 471, 137.
  2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793.
  3. Kim, D. (2003) Trends Pharmacol. Sci. 24, 648.
Scientific background

KCNK4 (also named TWIK-related arachidonic acid stimulated K+ channel, TRAAK or K2P4.1) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 16 members. These channels show little time- or voltage-dependence and are considered to be “leaky” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini.

K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK4 can be integrated to a K2P subfamily that includes K2P2.1 (TREK1) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid, high intracellular pH and mechanical stretch.

KCNK4 expression is largely confined to the brain in mouse samples while in humans it is also expressed in placenta and to a lesser degree in kidney, small intestine and prostate.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-KCNK4 (TRAAK) Antibody (#APC-108) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK4 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
More product citations
  1. de la Pena, E. et al. (2012) PLoS ONE 7, e52475.
  2. Wu, X. et al. (2012) Int. J. Urol. 19, 944.
  3. Hur, C.G. et al. (2009) Reproduction 137, 237.
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