KCNK5/TASK-2 Blocking Peptide (#BLP-PC037) is the original antigen used for immunization during Anti-KCNK5 (TASK-2) Antibody (#APC-037) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK5/TASK-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney membranes:
- Expression of KCNK5 in rat pancreasImmunohistochemical staining of rat pancreas was visualized using Anti-KCNK5 (TASK-2) Antibody (#APC-037), (1:50). Paraffin embedded sections of rat pancreas showing both endocrine (Islets of Langerhans) and exocrine areas. Strong and highly specific staining is evident only in endocrine cells (arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Substrate (Histofine, Nichirei Corp.) was used for the color reaction. Hematoxilin is used as the counterstain.
- (C)YEQLMNEYNKANSPKGT, amino acid residues 483-499 of human KCNK5 (Accession O95279).