KCNQ2 Blocking Peptide (#BLP-PC050) is the original antigen used for immunization during Anti-KCNQ2 Antibody (#APC-050) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of KCNQ2 transfected HEK-293 cells:
- Mouse optic nerve (Bekku, Y. et al. (2010) J. Neurosci. 30, 3113.).
- Western blot analysis of rat brain (lanes 1 and 2), mouse brain (lanes 3 and 4), human KCNQ2 transfected HEK-293 cells (lanes 5 and 7) and human brain neuroblastoma SH-SY5Y cell line (lanes 6 and 8) lysates:
- Expression of KCNQ2 in rat brainImmunohistochemical staining of free-floating frozen rat hippocampal CA1 region sections using Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP), (1:200). A. KCNQ2 staining (red) appears in the pyramidal layer (P). B. Nuclei are stained using DAPI as the counterstain (blue).
- (C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2 (Accession O88943).