Cat #: BLP-PC050
Type: Synthetic peptide
Form: Lyophilized powder
KCNQ2 Blocking Peptide (#BLP-PC050) is the original antigen used for immunization during Anti-KCNQ2 Antibody (#APC-050) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of KCNQ2 transfected HEK-293 cells:1. Anti-KCNQ2 Antibody (#APC-050), (1:200).
2. Anti-KCNQ2 Antibody, preincubated with KCNQ2 Blocking Peptide (#BLP-PC050).
- Mouse optic nerve (Bekku, Y. et al. (2010) J. Neurosci. 30, 3113.).
- Western blot analysis of rat brain (lanes 1 and 2), mouse brain (lanes 3 and 4), human KCNQ2 transfected HEK-293 cells (lanes 5 and 7) and human brain neuroblastoma SH-SY5Y cell line (lanes 6 and 8) lysates:1,3,5,6. Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP), (1:200).
2,4,7,8. Guinea pig Anti-KCNQ2 Antibody, preincubated with KCNQ2 Blocking Peptide (#BLP-PC050).
- Expression of KCNQ2 in rat brainImmunohistochemical staining of free-floating frozen rat hippocampal CA1 region sections using Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP), (1:200). A. KCNQ2 staining (red) appears in the pyramidal layer (P). B. Nuclei are stained using DAPI as the counterstain (blue).
- (C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2 (Accession O88943).
Accession (Uniprot) Number O88943
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.