Anti-KCNQ2 Antibody

KV7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2
    Cat #: APC-050
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2 (Accession O88943). Intracellular, C-terminus.
    Accession (Uniprot) Number O88943
    Gene ID 170848
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 15/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Specificity The antibody is specific for KCNQ2; it does not cross react with KCNQ3 or other QKT proteins. There are at least 9 recognized splice variants of rat KCNQ2. This antibody recognizes all except splice variants B and G.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of KCNQ2 transfected HEK-293 cells:
    1. Anti-KCNQ2 Antibody (#APC-050), (1:200).   
    2. Anti-KCNQ2 Antibody, preincubated with the control peptide antigen.
    Human SHSY-5Y cells (1:1000) (Mucha, M. et al. (2010) J. Neurosci. 30, 13235.).
    Immunohistochemistry
    Mouse optic nerve (Bekku, Y. et al. (2010) J. Neurosci. 30, 3113.).
    Immunocytochemistry
    Rat cortical neurons (1:500) (Peretz, A. et al. (2005) Mol. Pharmacol67, 1053.).
    References
    1. Kullmann, D.M. (2002) J. Mol. Brain 125, 1117.
    2. Robbins, J. (2001) Pharmacol. Ther. 90, 1.
    3. Wang, H.S. et al. (1998) Science 282, 1890.
    Scientific background

    The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels.  

    KCNQ family members can form either homo- or heteromultimeric channels with different functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor.  

    Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as Benign familial neonatal convulsions (BNFC).

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    KCNQ2/Caspr
    Anti-KCNQ2 Antibody
    Expression of KCNQ2 (KV7.2) in mouse optic nerve.Immunohistochemical staining of mouse optic nerve sections using Anti-KCNQ2 Antibody (#APC-050). KCNQ2 staining (green) appears in the Nodes of Ranvier. Caspr staining is shown in purple.Adapted from Bekku, Y. et al. (2010) J. Neurosci30, 3113. with permission of the Society for Neuroscience.

    Last update: 31/12/2018

    Anti-KCNQ2 Antibody (#APC-050) is a highly specific antibody directed against an epitope of the rat KV7.2 channel. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNQ2 from rat, human, and mouse samples.

    For research purposes only, not for human use
    Citations
    Western blot citations
    1. Rat cortical cell culture lysate (1:200).
      Figueiro-Silva, J. et al. (2015) J. Neurosci. 35, 5504.
    2. Human SHSY-5Y cells (1:1000).
      Mucha, M. et al. (2010) J. Neurosci. 30, 13235.
    Immunohistochemistry citations
    1. Guinea pig detrusor (1:200).
      Anderson, U.A. et al. (2013) Br. J. Pharmacol. 169, 1290.
    2. Rat skin sections (0.7 µg/ml).
      Reilly, J.M. et al. (2013) Pflugers Arch. 465, 1371.
    3. Mouse optic nerve.
      Bekku, Y. et al. (2010) J. Neurosci30, 3113.
    Immunocytochemistry citations
    1. Rat cortical culture (1:100).
      Figueiro-Silva, J. et al. (2015) J. Neurosci. 35, 5504.
    2. Rat cortical neurons (1:500).
      Peretz, A. et al. (2005) Mol. Pharmacol67, 1053.
    More product citations
    1. Etzioni, A. et al. (2011) J. Neurosci. 31, 14158.
    2. Kapfhamer, D. et al. (2010) J. Neurosci. 30, 8830.
    3. Devaux, J.J. (2010) J. Physiol. 588.23, 4719.
    4. Regev, N. et al. (2009) PLos ONE 4, e6586.
    5. Safiulina, V.F. et al. (2008) J. Physiol. 586.22, 5437.
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