KCNQ3 Blocking Peptide (#BLP-PC051) is the original antigen used for immunization during Anti-KCNQ3 Antibody (#APC-051) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of KCNQ3 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNQ3 Antibody (#APC-051), (1:200), followed by goat anti-rabbit-AlexaFluor-488. KCNQ3 immunoreactivity (green) appears in cells of the pyramidal layer (P, vertical arrows) and cells of the stratum radiatum (SR, horizontal arrows). Cell nuclei are stained with DAPI (blue).
- AEGEKKEDNRYSDLKTIIC, corresponding to amino acid residues 668-686 of rat KCNQ3 (Accession O88944).