Anti-KCNQ3 Antibody

KV7.3, Potassium voltage-gated channel subfamily KQT member 3, BFNC2
    Cat #: APC-051
    Alternative Name KV7.3, Potassium voltage-gated channel subfamily KQT member 3, BFNC2
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h
      • Peptide AEGEKKEDNRYSDLKTIIC, corresponding to amino acid residues 668-686 of rat KCNQ3 (Accession O88944). Intracellular, C-terminal.
        Anti-KCNQ3 Antibody
    Accession (Uniprot) Number O88944
    Gene ID O88944
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 18/19 amino acid residues identical.
    RRID AB_2040103.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, wb
    May also work in: ifc
      • Anti-KCNQ3 Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-KCNQ3 Antibody (#APC-051), (1:200).   
        2. Anti-KCNQ3 Antibody, preincubated with the control peptide antigen.
      • Anti-KCNQ3 Antibody
        Expression of KCNQ3 in rat hippocampus
        Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNQ3 Antibody (#APC-051), (1:200), followed by goat anti-rabbit-AlexaFluor-488. KCNQ3 immunoreactivity (green) appears in cells of the pyramidal layer (P, vertical arrows) and cells of the stratum radiatum (SR, horizontal arrows). Cell nuclei are stained with DAPI (blue).
      • Rat hippocampal neurons (1:100) (Rasmussen, H.B. et al. (2007) J. Cell Sci120, 953.).
      • The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels. KCNQ family members can form either homomultimeric or heteromultimeric channels with different functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor.  

        Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as benign familial neonatal convulsions (BFNC).

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-KCNQ3 Antibody
    Expression of KCNQ3 in guinea pig bladder detrusor smooth muscle.Immunohistochemical staining of guinea pig bladder detrusor smooth muscle sections using Anti-KCNQ3 Antibody (#APC-051). KCNQ3 staining (green) is detected in smooth muscle bundles (SM) and in interstitial cells (IC) adjacent to and between SM. Inset: pre-absorption control.Adapted from Anderson, U.A. et al. (2013) Br. J. Pharmacol. 169, 1290. with permission of John Wiley & Sons.

    Last update: 10/09/2019

    Anti-KCNQ3 Antibody (#APC-051) is a highly specific antibody directed against an epitope of the rat KV7.3 channel. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNQ3 from rat, human, and mouse samples.

    For research purposes only, not for human use
      • Western blot analysis of mouse hippocampus lysate (1:200). Tested in conditional knockout KCNQ3 brain lysate.
        Soh, H. et al. (2014) J. Neurosci. 34, 5311.
      • Rat brain lysate.
        Parrilla-Carrero, J. et al. (2018) J. Neurosci. 38, 4212.
      • CHO transfected cells.
        Ambrosino, P. et al. (2018) Mol. Neurobiol. 55, 7009.
      • Mouse brain lysate.
        Paz, R.M. et al. (2018) Neuropharmacology 137, 309.
      • HEK 293 transfected cells.
        Yau, M.C. et al. (2018) J. Gen. Physiol. 150, 1421.
      • Rat brain PVN membrane fraction(1:1000).
        Zhou, J.J. et al. (2017) Neuropharmacology 114, 67.
      • CHO transfected cells (1:1000).
        Miceli, F. et al. (2015) J. Neurosci. 35, 3782.
      • Mouse hippocampus lysate (1:200). Also tested in conditional knockout KCNQ3 brain lysate.
        Soh, H. et al. (2014) J. Neurosci. 34, 5311.
      • Mouse brain sections.
        Tirko, N.N. et al. (2018) Neuron 100, 593.
      • Mouse brain sections (1:100).
        Paz, R.M. et al. (2018) Neuropharmacology 137, 309.
      • Rat brainstem sections (1:100).
        Ghezzi, F. et al. (2018) J. Physiol. 596, 2611.
      • Rat brain sections.
        Zhou, J.J. et al. (2017) Neuropharmacology 114, 67.
      • Guinea pig detrusor (1:200).
        Anderson, U.A. et al. (2013) Br. J. Pharmacol. 169, 1290.
      • Rat hippocampal neurons (1:100).
        Rasmussen, H.B. et al. (2007) J. Cell Sci. 120, 953.
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