KCNQ5 Blocking Peptide (#BLP-PC155) is the original antigen used for immunization during Anti-KCNQ5 Antibody (#APC-155) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat hippocampus (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:
- Expression of KCNQ5 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNQ5 Antibody (#APC-155), (1:100). A. KCNQ5 (green) is expressed in hilar cells of the hippocampal dentate gyrus. B. Double-staining of KCNQ5 (green) with a neuronal marker (“Milli-Mark” from Millipore, red) reveals partial localization with hilar interneurons (arrow points at an example).
- (C)RGSQDFYPKWRESK, corresponding to amino acid residues 856-869 of mouse KCNQ5 (Accession Q9JK45).