Anti-KCNQ5 Antibody

KV7.5, Potassium voltage-gated channel subfamily KQT member 5, KQT-like 5, KvLQT5
Cat #: APC-155
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)RGSQDFYPKWRESK, corresponding to amino acid residues 856-869 of mouse KCNQ5 (Accession Q9JK45). Intracellular, C-terminus.
    Accession (Uniprot) Number Q9JK45
    Gene ID 226922
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, rat - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat hippocampus (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:
    1-3. Anti-KCNQ5 Antibody (#APC-155), (1:200).
    4-5. Anti-KCNQ5 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of KCNQ5 in rat hippocampus
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNQ5 Antibody (#APC-155), (1:100). A. KCNQ5 (green) is expressed in hilar cells of the hippocampal dentate gyrus. B. Double-staining of KCNQ5 (green) with a neuronal marker (“Milli-Mark” from Millipore, red) reveals partial localization with hilar interneurons (arrow points at an example).
    Immunocytochemistry
    Rat smooth muscle cells (1:100) (Morales-Cano, D. et al. (2015) Cardiovasc. Res. 106, 98.).
    References
    1. Brown, D.A. and Passmore, G.M. (2009) Br. J. Pharmacol. 156, 1185.
    2. Jentsch, T.J. (2000) Nat. Rev. Neurosci. 1, 21.
    3. Robbins, J. (2001) Pharmacol. Ther. 90, 1.
    4. Jensen, H.S. et al. (2005) Brain Res. Mol. Brain Res. 139, 52.
    5. Lerche, C. et al. (2000) J. Biol. Chem. 275, 22395.
    Scientific background

    KCNQ genes encode members of the KV7 K+ channels. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels. Five members belong to this family (KCNQ1-KCNQ5) of which KCNQ2 to KCNQ5 are expressed in the nervous system1-3. KCNQ5 is also expressed in visceral smooth muscle4 and skeletal muscle5.

    These channels form subunits of the low threshold voltage gated K+ channel. KCNQ2 and KCNQ3 mostly form heteromers to form the M current. KCNQ5 is known to contribute to this current1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-KCNQ5 Antibody
    Expression of KV7.5 (KCNQ5) in swine coronary artery.Immunohistochemical staining of swine coronary artery sections using Anti-KCNQ5 Antibody (#APC-155). KV7.5 staining (brown) is mostly detected in the intimal layer (I). Much lower staining is observed in medial layer (M).Adapted from Chen, X. et al. (2016) PLoS ONE 11, e0148569. with permission of PLoS.
    Last update: 03/03/2019

    Anti-KCNQ5 Antibody (#APC-155) is a highly specific antibody directed against an epitope of the mouse KV7.5 channel. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNQ5 from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
    Published figures using this product
    Expression of KCNQ1 (KV7.1) and KCNQ5 (KV7.5) in rat smooth muscle cells.
    Immunocytochemical staining of rat smooth muscle cells from LCA and RCA using Anti-KCNQ1 Antibody (#APC-022) and Anti-KCNQ5 Antibody (#APC-155) shows that the expression of both channels is higher in LCA than in RCA.
    Adapted from Morales-Cano, D. et al. (2015) with permission of the European Society of Cardiology.
    Western blot citations
    1. Rat brain lysate.
      Parrilla-Carrero, J. et al. (2018) J. Neurosci. 38, 4212.

    2. Mouse brain lysate.
      Paz, R.M. et al. (2018) Neuropharmacology 137, 309.

    Immunohistochemistry citations
    1. Rat brainstem sections (1:400).
      Ghezzi, F. et al. (2018) J. Physiol. 596, 2611.

    2. Mouse brain sections (1:100).
      Paz, R.M. et al. (2018) Neuropharmacology 137, 309.

    3. Swine coronary artery sections.
      Chen, X. et al. (2016) PLoS ONE 11, e0148569.
    Immunocytochemistry citations
    1. Rat smooth muscle cells (1:500).
      Morales-Cano, D. et al. (2015) Cardiovasc. Res. 106, 98.
    Related Products