Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNT2/Slick Blocking Peptide (#BLP-PC126) is the original antigen used for immunization during Anti-KCNT2 (Slick) Antibody (#APC-126) generation. The blocking peptide binds and ‘blocks’ Anti-KCNT2/Slick primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-KCNT2 (Slick) Antibody (#APC-126), (1:200).
3,4. Anti-KCNT2 (Slick) Antibody, preincubated with KCNT2/Slick Blocking Peptide (#BLP-PC126).
Properties
Sequence
- (C)KDVKDPGHHRSIHR, corresponding to amino acid residues 991-1004 of rat Slick (Accession Q6UVM4).
Accession (Uniprot) Number Q6UVM4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
