Anti-KCNT2 (Slick) Antibody

KCa4.2, Slo2.1 Na+ and Cl--activated K+ channel, Potassium channel subfamily T member 2
    Cat #: APC-126
    Alternative Name KCa4.2, Slo2.1 Na+ and Cl--activated K+ channel, Potassium channel subfamily T member 2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide (C)KDVKDPGHHRSIHR, corresponding to amino acid residues 991-1004 of rat Slick (Accession Q6UVM4). Intracellular, C-terminus.
        Anti-KCNT2 (Slick) Antibody
    Accession (Uniprot) Number Q6UVM4
    Gene ID 304827
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 13/14 amino acid residues identical; human - 10/14 amino acid residues identical.
    RRID AB_10613114.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, wb
    May also work in: ifc*, ih*, ip*
      • Anti-KCNT2 (Slick) Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
        1,2.  Anti-KCNT2 (Slick) Antibody (#APC-126), (1:200).
        3,4.  Anti-KCNT2 (Slick) Antibody, preincubated with the control peptide antigen.
      • Rat olfactory bulbs primary cultures. (Li, P. et al. (2017) eNeuro 4, e0114-17.2017.).
    References
      • KCa4.1 (Na+-activated K+ channel, Slack, Slo2.2, KCNT1) was originally cloned and named so due to its high similarity to the Slo genes1. Shortly after its discovery, KCa4.2 (Slick, Slo2.1, KCNT2), its sister channel, was also cloned2. Although KCa4.2 (like KCa4.1) is functionally a Na+-activated K+ channel (KNa), it is termed KCa by the IUPHAR nomenclature, due to its sequence homology to other KCa channels. Both channels are activated by high intracellular concentrations of Na+.

        Like Slack, Slick contains six transmembrane spanning domains, a P-region between transmembrane regions 5 and 6 and intracellular N- and C-termini. However, the N-terminal domain of Slick is significantly shorter than that of Slack. In addition, contrary to Slack, Slick is regulated by ATP as it has an ATP binding site in its C-terminal domain2. ATP binding reduces the activity of the channel and mutations of this site abolish the inhibitory effect2. Both channels are regulated by intracellular Cl- ions, but Slick displays higher sensitivity3. Also, the overall electrical characteristics of Slick channels are different from those of Slack; Slick is rapidly activated in response to depolarization, and also has a basal level of activity in the absence of Na+.  

        Both channels are highly expressed in the brain with overlapping expression. Slick is found in the midbrain, brainstem, and hippocampus and throughout the neocortex. This KNa channel is also detected in the auditory neurons in the brainstem. Detection of Slick was also found in the heart, although at much lower levels2.

        Many different functions have been attributed to KNa channels including action potential repolarization, slow after-hyperpolarization, burst firing and adaptation after repetitive firing3. These channels also contribute to the response of neurons to hypoxia.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-KCNT2 (Slick) AntibodyExpression of Slick (Slo2.1) channel in rat olfactory bulb.Immunocytochemical staining of dissociated rat olfactory bulbs primary cultures using Anti-KCNT2 (Slick) Antibody (#APC-126). A. Slick staining (green). B. GABA(B) R1 receptor staining (red). C. Merged image.Adapted from Li, P. et al. (2017) eNeuro 4, e0114-17.2017. with permission of the Society for Neuroscience.

    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of rat KCNT2. Anti-KCNT2 (Slick) Antibody (#APC-126) can be used in western blot and immunocytochemistry applications. It has been designed to recognize KCNT2 from rat, human, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Rat olfactory bulbs primary cultures.
        Li, P. et al. (2017) eNeuro 4, e0114-17.2017.
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