Kir2.1/KCNJ2 (extracellular) Blocking Peptide (#BLP-PC159) is the original antigen used for immunization during Anti-Kir2.1/KCNJ2 (extracellular) Antibody (#APC-159) generation. The blocking peptide binds and ‘blocks’ Anti-Kir2.1/KCNJ2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and mouse heart lysate (lanes 3 and 6):
- Expression of Kir2.1 in mouse midbrain.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Kir2.1/KCNJ2 (extracellular) Antibody (#APC-159), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the dorsal midbrain region showed immunoreactivity (green) in multipolar neurons (vertical arrow). B. Pre-incubation of the antibody with Kir2.1/KCNJ2 (extracellular) Blocking Peptide (BLP-PC159), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)DLDASKESKA(S)VSE, corresponding to amino acid residues 112 - 125 of rat Kir2.1 (Accession Q64273).