Anti-Kir2.1 (KCNJ2) (extracellular) Antibody

Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3
    Cat #: APC-159
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)DLDASKESKA(S)VSE, corresponding to amino acid residues 112 - 125 of rat Kir2.1 (Accession Q64273). Extracellular loop.
    Accession (Uniprot) Number Q64273
    Gene ID 29712
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, dog, pig - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical; mouse - 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, lci, wb
    May also work in: ih, ip
    Western blot
    Western blot analysis of rat brain lysate (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and mouse heart lysate (lanes 3 and 6):
    1-3. Anti-Kir2.1 (KCNJ2) (extracellular) Antibody (#APC-159), (1:200).
    4-6. Anti-Kir2.1 (KCNJ2) (extracellular) Antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry of live intact human THP-1 monocytic leukemia cells:
    ___ Cell alone
    ___ Cells + goat-anti-rabbit-Alexa-488
    ___ Cells + Anti-Kir2.1 (KCNJ2) (extracellular) Antibody (#APC-159), 3 µl + goat-anti-rabbit-AlexaFluor-488 secondary antibody.
    Live cell imaging / Immunocytochemistry
    Expression of Kir2.1 in rat RBL-2H3 cells
    Immunocytochemical staining of live intact rat RBL-2H3 basophilic leukemia cells. A. Extracellular staining of cells using Anti-Kir2.1 (KCNJ2) (extracellular) Antibody (#APC-159), (1:50), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells.
    References
    1. Hibino, H. et al. (2010) Physiol. Rev. 90, 291.
    Scientific background

    Inwardly rectifying K+ (Kir) channels allow K+ to move more easily in, rather than out, of the cell. They have diverse physiological functions depending on their type and their location. There are seven Kir channel subfamilies that can be classified into four functional groups.

    Kir2.1 belongs to the Kir2.x subfamily. The primary structure of Kir2.1 is comprised of two putative membrane-spanning domains (TM1 and TM2) linked by an extracellular pore-forming region (H5) and cytoplasmic amino and carboxy terminal domains. The H5 region serves as the “ion-selectivity filter” that is shared with other K+-selective ion channels with the signature sequence T-X-G-Y(F)-G.

    Although initially thought to form only homomeric complexes, it is now known that Kir2.1 can function as a heterotetramer with other Kir2.x subunits both in vitro and in vivo. 

    After synthesis in the endoplasmic reticulum, Kir2.1 is transported to the Golgi apparatus for post-translational modification, and then transported to the cell surface. Sequences in the NH2-terminal region have been identified as responsible for Golgi export.

    Kir2.1 is expressed in skeletal and cardiac muscle and contributes to the establishment of highly negative resting potential and long-lasting action potential plateau in various cells including cardiac myocytes. Inward rectification of Kir2.1 is caused by intracellular ions such as Mg2+ and polyamines.

    Andersen syndrome is caused by dysfunction of Kir2.1. Patients with Andersen syndrome exhibit cardiac arrhythmias reminiscent of long Q-T syndrome, periodic paralysis, and dysmorphic bone structure in the face and fingers.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/11/2018

    Anti-Kir2.1 (KCNJ2) (extracellular) Antibody (#APC-159) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging and live cell flow cytometry. It has been designed to recognize Kir2.1 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
    Related Products
      • Products for control experiments
        1. Anti-Kir2.1 (KCNJ2) Antibody (#APC-026). This antibody recognizes a different epitope and thus can also be used as a control for detecting Kir2.1 channel.
      • Antibodies for immuno-colocalization experiments
        1. Guinea pig Anti-Kir2.1 (KCNJ2) Antibody (#AGP-044) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.