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4-6. Anti-Kir2.1 (KCNJ2) (extracellular) Antibody, preincubated with the control peptide antigen.
___ Cells + goat-anti-rabbit-Alexa-488
___ Cells + Anti-Kir2.1 (KCNJ2) (extracellular) Antibody (#APC-159), 3 µl + goat-anti-rabbit-AlexaFluor-488 secondary antibody.
- Hibino, H. et al. (2010) Physiol. Rev. 90, 291.
Inwardly rectifying K+ (Kir) channels allow K+ to move more easily in, rather than out, of the cell. They have diverse physiological functions depending on their type and their location. There are seven Kir channel subfamilies that can be classified into four functional groups.
Kir2.1 belongs to the Kir2.x subfamily. The primary structure of Kir2.1 is comprised of two putative membrane-spanning domains (TM1 and TM2) linked by an extracellular pore-forming region (H5) and cytoplasmic amino and carboxy terminal domains. The H5 region serves as the “ion-selectivity filter” that is shared with other K+-selective ion channels with the signature sequence T-X-G-Y(F)-G.
Although initially thought to form only homomeric complexes, it is now known that Kir2.1 can function as a heterotetramer with other Kir2.x subunits both in vitro and in vivo.
After synthesis in the endoplasmic reticulum, Kir2.1 is transported to the Golgi apparatus for post-translational modification, and then transported to the cell surface. Sequences in the NH2-terminal region have been identified as responsible for Golgi export.
Kir2.1 is expressed in skeletal and cardiac muscle and contributes to the establishment of highly negative resting potential and long-lasting action potential plateau in various cells including cardiac myocytes. Inward rectification of Kir2.1 is caused by intracellular ions such as Mg2+ and polyamines.
Andersen syndrome is caused by dysfunction of Kir2.1. Patients with Andersen syndrome exhibit cardiac arrhythmias reminiscent of long Q-T syndrome, periodic paralysis, and dysmorphic bone structure in the face and fingers.
Species reactivity key:
Anti-Kir2.1 (KCNJ2) (extracellular) Antibody (#APC-159) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging and live cell flow cytometry. It has been designed to recognize Kir2.1 from human, rat, and mouse samples.
Products for control experiments
- Anti-Kir2.1 (KCNJ2) Antibody (#APC-026). This antibody recognizes a different epitope and thus can also be used as a control for detecting Kir2.1 channel.
Antibodies for immuno-colocalization experiments
- Guinea pig Anti-Kir2.1 (KCNJ2) Antibody (#AGP-044) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.
- Blockers/Antagonists: small molecules