Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kir2.2/KCNJ12 Blocking Peptide (#BLP-PC042) is the original antigen used for immunization during Anti-Kir2.2 (KCNJ12) Antibody (#APC-042) generation. The blocking peptide binds and ‘blocks’ Anti-Kir2.2/KCNJ12 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200).
3,4. Anti-Kir2.2 (KCNJ12) Antibody, preincubated with Kir2.2/KCNJ12 Blocking Peptide (#BLP-PC042).
Expression of Kir2.2 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Kir2.2 immunoreactivity (green) appeared in Bergmann glial fibers (right-pointing arrows) and outlines of molecular layer neurons (left-pointing arrows). B. Pre-incubation of the antibody with Kir2.2/KCNJ12 Blocking Peptide (BLP-PC042), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer.
Properties
Sequence
- (C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188).
Accession (Uniprot) Number P52188
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
