Kir2.2/KCNJ12 Blocking Peptide (#BLP-PC042) is the original antigen used for immunization during Anti-Kir2.2 (KCNJ12) Antibody (#APC-042) generation. The blocking peptide binds and ‘blocks’ Anti-Kir2.2/KCNJ12 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:
- Expression of Kir2.2 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Kir2.2 immunoreactivity (green) appeared in Bergmann glial fibers (right-pointing arrows) and outlines of molecular layer neurons (left-pointing arrows). B. Pre-incubation of the antibody with Kir2.2/KCNJ12 Blocking Peptide (BLP-PC042), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer.
- (C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188).