Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035) is the original antigen used for immunization during Anti-Kir4.1 (KCNJ10) Antibody (#APC-035) generation. The blocking peptide binds and ‘blocks’ Anti-Kir4.1/KCNJ10 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6):
- Expression of Kir4.1 in rat hippocampal dentate gyrusImmunohistochemical staining of rat frozen brain sections using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:300). A. Kir4.1 staining (green) reveals Kir4.1 clusters in the molecular layer (Mol). B. The same section was stained with mouse anti-glial fibrillary acidic protein (GFAP), (red) showing astrocyte profiles. C. Merge of Kir4.1 and GFAP images reveals colocalization on some branches of astrocytic fibers. In addition, Kir4.1 is expressed in the fine ramification of astrocytic fibers, thus creating a “halo-like” cloud around the GFAP astrocytic outline. DAPI (blue) counterstain reveals the outline of the dentate granule layer.
- (C)KLEE SLREQ AEKEG SALSV R, corresponding to amino acid residues 356-375 of rat Kir4.1 (Accession P49655).