Kir5.1 Blocking Peptide (#BLP-PC123) is the original antigen used for immunization during Anti-Kir5.1 Antibody (#APC-123) generation. The blocking peptide binds and ‘blocks’ Anti-Kir5.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes:
- Western blot analysis of mouse brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:
- Expression of Kir5.1 in rat kidneyImmunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir5.1 Antibody (#APC-123) (1:100). Kir5.1 (brown staining) is expressed in both proximal tubules (PT) and distal tubules (DT) in the renal cortex. Note that collecting ducts (CD) are less stained while glomeruli (G) are negative. Hematoxilin is used as the counterstain.
- Human brain sections (1:500) (Schirmer, L. et al. (2014) Ann. Neurol. 75, 810.).
- HDVLEVKRKYYKVNC, corresponding to amino acid residues 311-325 of rat KCNJ16 (Accession P52191).