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Anti-Kir5.1 Antibody

Inward rectifier potassium channel 16, KCNJ16, BIR9

Cat #: APC-123
Alternative Name Inward rectifier potassium channel 16, KCNJ16, BIR9
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide HDVLEVKRKYYKVNC, corresponding to amino acid residues 311-325 of rat KCNJ16 (Accession P52191). Intracellular, C-terminal.
Accession (Uniprot) Number P52191
Gene ID 29719
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human 14/15 amino acid residues-identical.
RRID AB_2039941.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes:
    Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes:
    1,2. Anti-Kir5.1 Antibody (#APC-123), (1:200).
    3,4. Anti-Kir5.1 Antibody, preincubated with Kir5.1 Blocking Peptide (#BLP-PC123).
  • Western blot analysis of mouse brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:
    Western blot analysis of mouse brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:
    1,2. Anti-Kir5.1 Antibody (#APC-123), (1:200).
    3,4. Anti-Kir5.1 Antibody, preincubated with Kir5.1 Blocking Peptide (#BLP-PC123).
Immunohistochemistry
  • Expression of Kir5.1 in rat kidney
    Expression of Kir5.1 in rat kidney
    Immunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir5.1 Antibody (#APC-123) (1:100). Kir5.1 (brown staining) is expressed in both proximal tubules (PT) and distal tubules (DT) in the renal cortex. Note that collecting ducts (CD) are less stained while glomeruli (G) are negative. Hematoxilin is used as the counterstain.
  • Human brain sections (1:500) (Schirmer, L. et al. (2014) Ann. Neurol. 75, 810.).
References
  1. Bond, C.T. et al. (1994) Rec. Channels 2, 183.
  2. Hebert, S.C. et al. (2005) Physiol. Rev. 85, 319.
  3. Tanemoto, M. et al. (2002) Neuron 34, 387.
Scientific background

Kir5.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.

The family’s protein topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heteromers.

Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.1

Kir5.1 can co-assemble with other Kir channels such as Kir2.1Kir4.1 and Kir4.2. In fact, heteromeric Kir4.1/Kir5.1 form the basolateral small-conductance K+ channel in distal nephron segments that is responsible for generating the basolateral membrane potential that determines the magnitude and direction of K+ diffusion from cell to peritubular fluid in the nephron.2

The Kir5.1 homomeric channel was largely thought to be non-functional, although recent evidence demonstrates that Kir5.1 homomers can form functional channels when co-expressed with the anchor protein PSD-95 in the brain where it is abundantly expressed.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-Kir5.1 AntibodyExpression of Kir4.1 and Kir5.1 channels in rat brainstem.Immunohistochemical staining of rat brainstem sections using Anti-Kir4.1 (KCNJ10) Antibody (#APC-035) and Anti-Kir5.1 Antibody (#APC-123) showed that both channels are expressed (green). Kir4.1 staining is limited to astrocytes, while that of Kir5.1 is detected in 5-HT neurons together with the 5-HT neuronal marker TPH (red).Adapted from Puissant, M.M. et al. (2017) Front. Cell. Neurosci. 11, 34. with permission of Frontiers.
Last update: 25/07/2021

Anti-Kir5.1 Antibody (#APC-123) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir5.1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Western blot citations
  1. Rat brain lysate (1:100).
    Puissant, M.M. et al. (2017) Front. Cell. Neurosci. 11, 34.
  2. Mouse brain lysate (1:200).
    Zhang, X. et al. (2011) Am. J. Physiol. 301, C729.
Immunohistochemistry citations
  1. Mouse brain sections.
    Brasko, C. et al. (2017) Brain Struct. Funct. 222, 41.
  2. Rat brainstem sections (1:1000).
    Puissant, M.M. et al. (2017) Front. Cell. Neurosci. 11, 34.
  3. Human brain sections (1:500).
    Schirmer, L. et al. (2014) Ann. Neurol. 75, 810.

Specifications

Scientific Background

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