Kir6.1/KCNJ8 Blocking Peptide (#BLP-PC105) is the original antigen used for immunization during Anti-Kir6.1 (KCNJ8) Antibody (#APC-105) generation. The blocking peptide binds and ‘blocks’ Anti-Kir6.1/KCNJ8 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat heart membranes:
- Western blot analysis of rat cortex lysate:
- Expression of Kir6.1 in rat uterusImmunohistochemical stain of pregnant rat longitudinal section of the myometrium using Anti-Kir6.1 (KCNJ8) Antibody (#APC-105), (1:50). Strong and specific staining is evident in smooth muscles cells both in the myometrium (blue arrow) and muscular layers of blood vessels (green arrow). Peroxidase reaction with DAB were used for the color reaction. Hematoxilin is used as the counterstain.
- Mouse brain sections (1:100) (Parsons, M.P. and Hirasawa, M. (2010) J. Neurosci. 30, 8061.).
- (C)KRNSMRRNNSMRRSN, corresponding to amino acid residues 382-396 of rat Kir6.1 (Accession Q63664).