Kir6.2 Blocking Peptide (#BLP-PC020) is the original antigen used for immunization during Anti-Kir6.2 Antibody (#APC-020) generation. The blocking peptide binds and ‘blocks’ Anti-Kir6.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat pancreas membranes:
- Expression of Kir6.2 in rat pancreasImmunohistochemical staining of rat pancreas using Anti-Kir6.2 Antibody (#APC-020). A. Strong granular staining in a number of cells within the Islets of Langerhans is readily detected (red). B. The negative control slide shows no staining.
- Human placenta sections (Lybaert, P. et al. (2013) Placenta 34, 467.).
- Western blot analysis of rat heart membranes (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):
- Western blot analysis of human SHSY-5Y neuroblastoma cell lysate:
- Expression of Kir6.2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:300), followed by goat-anti-guinea pig-Cy3 antibody (red staining). Kir6.2 staining appears in the hilus region in neuronal outlines (arrows). Nuclei are stained with DAPI (blue).
- (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673).