Anti-Kir6.2 Antibody

Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3
    Cat #: APC-020
    Alternative Name Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673). Intracellular, C-terminal part.
        Anti-Kir6.2 Antibody
    Accession (Uniprot) Number P70673
    Gene ID 83535
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 12/14 amino acid residues identical.
    RRID AB_2040124.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity The antibody is specific for Kir6.2 and does not cross react with Kir6.1.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 2 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, wb
      • Anti-Kir6.2 Antibody
        Western blot analysis of rat pancreas membranes:
        1. Anti-Kir6.2 Antibody (#APC-020), (1:200).   
        2. Anti-Kir6.2 Antibody, preincubated with the control peptide antigen.
      • Mouse heart lysate (Li, J. et al. (2010) J. Biol. Chem. 285, 28723.).
      • Anti-Kir6.2 Antibody
        Expression of Kir6.2 in rat pancreas
        Immunohistochemical staining of rat pancreas using Anti-Kir6.2 Antibody (#APC-020). A. Strong granular staining in a number of cells within the Islets of Langerhans is readily detected (red). B. The negative control slide shows no staining.
        Human placenta sections (Lybaert, P. et al. (2013) Placenta 34, 467.).
      • Mouse ciliary cells (1:100) (Ohba, T. et al. (2013) J. Pharmacol. Exp. Ther. 347, 145.)
      • Mouse endothelial cells. Also tested in Kir6.2-/- mice (Milovanova, T. et al. (2005) Am. J. Physiol. 290, C66.).

    References
      • Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir channels can be modulated by a variety of intracellular agents such as protons, GTP-binding proteins and adenine nucleotides.  

        The ATP-sensitive channel (KATP) is especially important since it couples cellular metabolism (intracellular ATP levels) with cell excitability. KATP channels have been described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle.  

        The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea receptor (SUR) subunit.  

        The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and   SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes. 

        Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk of developing type-2 diabetes.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

     
    Knockout validation of Anti-Kir6.2 Antibody in mouse endothelial cells.Immunocytochemical staining of mouse endothelial cells using Anti-Kir6.2 Antibody (#APC-020). A. Kir6.2 staining (red) in wild type cells. B. Kir6.2 immunoreactivity is not detected in Kir6.2-/- cells.Adapted from Milovanova, T. et al. (2005) Am. J. Physiol. 290, C66. with permission of The American Physiological Society.

    Last update: 09/12/2019

    Anti-Kir6.2 Antibody (#APC-020) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Kir6.2 from human, rat, and mouse samples. The antibody is specific for Kir6.2 and does not cross react with Kir6.1.

    For research purposes only, not for human use
    Citations
      • Anti-Kir6.2 Antibody
        Expression of Kir6.2 in rat suprachiasmatic nucleus sections
        Immunohistochemical staining of rat suprachiasmatic nucleus sections (SCN) using Anti-Kir6.2 Antibody (#APC-020). Kir6.2 staining (green) colocalizes with Arginine-Vasopressin (AVP) staining (red) in the soma (middle panel) and in varicosities along the process (right panel).
        Adapted from Yang, J.J. et al. (2017) Sci. Rep. 7, 640. with permission of SPRINGER NATURE.
        Anti-Kir6.2 Antibody
        Expression of Kir6.2 in mouse cardiomyocytes.
        Immunocytochemical staining of mouse cardiomyocytes using Anti-Kir6.2 Antibody (#APC-020).
        Adapted from Li, J. et al. (2010) J. Biol. Chem. 285, 28723. with permission of The American Society for Biochemistry and Molecular Biology.
      • Western blot analysis, immunocytochemistry, and indirect flow cytometry of mouse endothelial cells. Tested in Kir6.2-/- mice.
        Milovanova, T. et al. (2005) Am. J. Physiol. 290, C66.
      • Human myometrium tissue lysate.
        Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.
      • Rat immortalized chromaffin (MAH) cell lysate.
        Salman, S. et al. (2014) Am. J. Physiol. 307, C266.
      • Human placenta and pancreas lysate.
        Lybaert, P. et al. (2013) Placenta 34, 467.
      • Rat uterine tissue lysate.
        Novakovic, R. et al. (2013) J. Physiol. Pharmacol. 64, 795.
      • Rat primary adrenomedullary chromafin cells (AMCs) (1:1000).
        Salman, S. et al. (2013) J. Physiol. 591, 515.
      • Rat trigeminal ganglia lysate (1:500).
        Niu, K. et al. (2011) Neuroscience 180, 344.
      • Rat heart lysate (1:200).
        Yang, Z.W. et al. (2011) Acta Pharmacol. Sin. 32, 194.
      • Mouse heart lysate.
        Li, J. et al. (2010) J. Biol. Chem. 285, 28723.
      • Rat DRG lysate (2010).
        Zoga, V. et al. (2010) Mol. Pain 6, 1.
      • Mouse endothelial cell lysate. Also tested in Kir6.2-/- mice.
        Milovanova, T. et al. (2005) Am. J. Physiol. 290, C66.
      • Mouse heart lysate.
        Li, J. et al. (2010) J. Biol. Chem. 285, 28723.
      • Rat brain sections.
        Yang, J.J. et al. (2017) Sci. Rep. 7, 640.
      • Human myometrium sections (1:50).
        Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.
      • Human placenta sections.
        Lybaert, P. et al. (2013) Placenta 34, 467.
      • Human artery tissues (1:50).
        Gojkovic-Bukarica, L. et al. (2011) Eur. J. Pharmacol. 654, 266.
      • Human HSV and HIMA tissues (1:50).
        Gojkovic-Bukarica, L.C. et al. (2011) J. Cardiovasc. Pharmacol. 57, 648.
      • Rat trigeminal ganglia sections (1:1500).
        Niu, K. et al. (2011) Neuroscience 180, 344.
      • Rat heart sections (1:100).
        Yang, Z.W. et al. (2011) Acta Pharmacol. Sin. 32, 194.
      • Rat DRG sections (1:500).
        Zoga, V. et al. (2010) Mol. Pain 6, 1.
      • Mouse ciliary cells (1:100).
        Ohba, T. et al. (2013) J. Pharmacol. Exp. Ther. 347, 145.
      • Mouse cardiomyocytes.
        Li, J. et al. (2010) J. Biol. Chem. 285, 28723.
      • Mouse endothelial cells. Also tested in Kir6.2-/- mice.
        Milovanova, T. et al. (2005) Am. J. Physiol. 290, C66.
      • Mouse endothelial cells. Also tested in Kir6.2-/- mice.
        Milovanova, T. et al. (2005) Am. J. Physiol. 290, C66.
      • Xia, F. et al. (2004) J. Biol. Chem. 279, 24685.
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