Cat #: BLP-KR001
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of Jurkat (lanes 1 and 4), HL-60 (lanes 2 and 5), MCF-7 (lanes 3 and 6) and rat brain (lanes 7 and 8) lysates:1,2,3,7. Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (1:500).
4,5,6,8. Anti-KISS1 Receptor (GPR54) (extracellular) Antibody, preincubated with KISS1 Receptor/GPR54 (extracellular) Blocking Peptide (#BLP-KR001).
- Expression of KISS1R in rat pancreasImmunohistochemical staining of paraffin embedded section of rat pancreas using Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (1:100). KISS1R staining (brown) appears in Isles of Langerhans (IL). Hematoxilin is used as the counterstain.
- Expression of KISS1R in rat cerebellumImmunohistochemical staining of frozen rat cerebellum section using Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (1:100). A. KISS1R (green) was expressed particularly in Purkinje cell bodies (arrows). B. Staining with mouse anti-parvalbumin (red) detected Purkinje cells and interneurons in the molecular layer. C. Merge of the two images demonstrates that the staining was restricted to Purkinje cells.
- (C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R (Accession Q969F8).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 4 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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