Kv1.6/KCNA6 Blocking Peptide (#BLP-PC003) is the original antigen used for immunization during Anti-KV1.6 (KCNA6) Antibody (#APC-003) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.6/KCNA6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Rat brain sections (see also 1,2 in publications using this product). Mouse cerebellum (1:350) (Kleopa, K.A. et al. (2006) Brain 129, 1570.).
- GST fusion protein with the sequence NYFYHRETEQEEQGQYTHVTCGQPTPDLKATDNGLGKPDFAEASRERRSSYLPTPHRAYAEKRMLTEV, corresponding to amino acid residues 463-530 of rat KV1.6 (Accession P17659).