Anti-KV1.6 Antibody

KCNA6
    Cat #: APC-003
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    GST fusion protein with a sequence NYFYHRETEQEEQGQYTHVTCGQPTPDLKATDNGLGKPDFAEAS
    RERRSSYLPTPHRAYAEKRMLTEV, corresponding to amino acid residues 463-530 of rat KV1.6 (Accession P17659), (MW: 35 kDa.). Intracellular, C-terminus.
    Accession (Uniprot) Number P17659
    Gene ID P17659
    Peptide confirmation Confirmed by DNA sequence and SDS-PAGE.
    Homology Mouse - 67/68 amino acid residues identical; human - 63/68 amino acid residues identical.
    Purity The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and from antibodies cross-reactive to other Kv1 by affinity chromatography on immobilized Kv1.1-GST and Kv1.4-GST, and then the antibody was affinity purified on immobilized Kv1.6-GST.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 3 µg fusion protein per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain membranes:
    1. Anti-KV1.6 Antibody (#APC-003), (1:200).
    2. Anti-KV1.6 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Rat brain sections (see also 1,2 in publications using this product). Mouse cerebellum (1:350) (Kleopa, K.A. et al. (2006) Brai129, 1570.).
    Immunocytochemistry
    HeLa transfected cells (1:200) (Kleopa, K.A. et al. (2006) Brai129, 1570.).
    References
    1. Grupe, A. et al. (1990) EMBO J. 9, 1749.
    2. Gutman, G. A. et al. (2005) Pharmacol. Rev57, 473.
    3. Bogin, O. (2006) Modulator 21, 28.
    Scientific background

    KV1.6 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.6 was first cloned from human brain.1 Eight Shaker-related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2

    A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.

    The structure of KV1.6 channel is similar to all KV channels and includes six membrane spanning helixes creating a voltage sensor domain and a pore domain.2

    The channel is expressed in neurons and other supporting cells in the brain, in cardiac and smooth muscle tissue as well as in ovary and testis2 and its activity influences the membrane potential and excitability of expressing cells.

    KV1.6 channels are sensitive to low doses of TEA (7 mM) and high doses of 4-AP (1.5 mM), the “classical” non-selective potassium channel blockers.

    Several toxins from snakes, scorpions and sea anemones venoms are potent blockers (affecting the channels in the nanomolar range) of KV1.6 channels. Among these the most potent and selective are α-Dendrotoxin (#D-350), (9-25 nM) and δ-Dendrotoxin (#D-380), (23 nM), Agitoxin-2 (#STA-420), (0.036 nM) Hongotoxin-1 (#RTH-400), (6 nM), Margatoxin (#STM-325), (5 nM) and Stichodactyla Toxin (#STS-400), (0.16 nM).3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 06/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.6. Anti-KV1.6 Antibody (#APC-003) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KV1.6 from human, rat and mouse samples.

    For research purposes only, not for human use
    Citations
    Immunohistochemistry citations
    1. Mouse brain sections (1:400).
      Blosa, M. et al. (2015) J. Physiol. 593, 4341.
    2. Human artery tissues (1:50).
      Gojkovic-Bukarica, L. et al. (2011) Eur. J. Pharmacol.  654, 266.
    More product citations
    1. Hao, J. et al. (2013) Neuron 77, 899.
    2. Kleopa, K.A. et al. (2006) Brai129, 1570.
    3. Chittajallu, R. et. al. (2002) PNAS 99, 2350.
    4. Chung, Y.H. et al. (2000) Brain Res. 875, 164.
    5. Smart, S.L. et al. (1997) Glia 20, 127.
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