Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv12.1/KCNH8 Blocking Peptide (#BLP-PC113) is the original antigen used for immunization during Anti-KV12.1 (KCNH8) Antibody (#APC-113) generation. The blocking peptide binds and ‘blocks’ Anti-Kv12.1/KCNH8 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cortex lysate:1. Anti-KV12.1 (KCNH8) Antibody (#APC-113), (1:200).
2. Anti-KV12.1 (KCNH8) Antibody, preincubated with Kv12.1/KCNH8 Blocking Peptide (#BLP-PC113).
Properties
Sequence
- EDKKEDRAKGRSRAG(C), corresponding to amino acid residues 143-157 of rat KCNH8 (Accession Q9QWS8).
Accession (Uniprot) Number Q9QWS8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
