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Anti-KV12.1 (KCNH8) Antibody

Potassium voltage-gated channel subfamily H member 8, Ether-a-go-go-like potassium channel 3, ELK channel 3, Elk1, hElk1

Cat #: APC-113
Alternative Name Potassium voltage-gated channel subfamily H member 8, Ether-a-go-go-like potassium channel 3, ELK channel 3, Elk1, hElk1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
May also work in: h*, m*
Immunogen
  • Peptide EDKKEDRAKGRSRAG(C), corresponding to amino acid residues 143-157 of rat KCNH8 (Accession Q9QWS8). Intracellular, N-terminal part.
Accession (Uniprot) Number Q9QWS8
Gene ID 246325
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse -14/15 amino acid residues identical; human -13/15 amino acid residues identical.
RRID AB_2039939.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat cortex lysate:
    Western blot analysis of rat cortex lysate:
    1. Anti-KV12.1 (KCNH8) Antibody (#APC-113), (1:200).
    2. Anti-KV12.1 (KCNH8) Antibody, preincubated with Kv12.1/KCNH8 Blocking Peptide (#BLP-PC113).
References
  1. Shi, W. et al. (1998) J. Physiol. 511, 675.
  2. Zou, A. et al. (2003) Am. J. Physiol. Cell. Physiol. 285, C1356.
Scientific background

KV12.1 is a voltage-gated K+ channel that belongs to the ether-a-go-go (EAG) family. The EAG family of voltage-gated K+ channels can be subdivided into three distinct groups based on sequence homology. These are the eag (KV10) with two members, the eag-related channels (erg or KV11) and the eag-like K+ channels (elk or KV12) with three members each.

As with all members of the family, KV12.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C- termini. The functional channel is a tetramer composed of four subunits. It has been suggested that the KV12 subfamily members can form functional heteromultimers within the subfamily.

KV12.1 channel distribution appears to be mainly confined to the central nervous system with some expression in peripheral organs such as the testis, colon and lung.

The physiological function of the KV12.1 channel is yet to be established, although a role in the modulation of overall excitability of neurons has been suggested.  

Currently, there isn’t much pharmacological data concerning the modulation of the KV12.1 channel except for blocking of the channel by Ba2+.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 24/01/2021

Alomone Labs is pleased to offer Anti-KV12.1 (KCNH8) Antibody (#APC-113) that is directed against a highly conserved epitope of the rat protein. The antibody will also likely recognize human and mouse samples.

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For research purposes only, not for human use

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